Integrative Proteomics

00 preface_ Integrative Proteomics......Page 1
Part 1_ Proteomics - Historical Review......Page 11
01_Strategies for Protein Separation\r......Page 13
02_Evolution of Proteomic Methods for\rAnalysis of Complex Biological Samples –\rImplications for Personalized Medicine\r......Page 39
Part 2_ Sample Preparation......Page 55
03_Proteomic Analyses of Cells\rIsolated by Laser Microdissection\r......Page 57
04_A Critical Review of Trypsin Digestion\rfor LC-MS Based Proteomics\r......Page 83
05_Simple and Rapid Proteomic Analysis\rby Protease-Immobilized Microreactors\r......Page 103
06_Labeling Methods in\rMass Spectrometry Based\rQuantitative Proteomics\r......Page 121
Part 3_ 2D Gel Electrophoresis and Databases......Page 143
07_Preparation of Protein Samples\rfor 2-DE from Different Cotton Tissues\r......Page 145
08_2D-PAGE Database for\rStudies on Energetic Metabolism of the\rDenitrifying Bacterium Paracoccus denitrificans\r......Page 151
Part 4_ Subproteomes Analyses......Page 167
09_Targeted High-Throughput Glycoproteomics\rfor Glyco-Biomarker Discovery\r......Page 169
10_Recent Advances in Glycosylation\rModifications in the Context of\rTherapeutic Glycoproteins\r......Page 193
11_Detection of Protein Phosphorylation\rby Open-Sandwich Immunoassay\r......Page 207
12_Phosphoproteomics: Detection, Identification\rand Importance of Protein Phosphorylation\r......Page 225
13_Proteome Kinetics: Coupling the\rAdministration of Stable Isotopes\rwith Mass Spectrometry-Based Analyses\r......Page 243
14_Dynamics of Protein Complexes\rTracked by Quantitative Proteomics\r......Page 267
15_Proteomics Analysis of\rKinetically Stable Proteins\r......Page 291
16_Vinyl Sulfone:\rA Multi-Purpose Function in Proteomics\r......Page 311
17_Gel-Free Proteome Analysis\rIsotopic Labelling Vs. Label-Free\rApproaches for Quantitative Proteomics\r......Page 337
18_Quantitative Proteomics Using\riTRAQ Labeling and Mass Spectrometry\r......Page 357
19_Functional Proteomics:\rMapping Lipid-Protein Interactomes\r......Page 373
20_Protein Thiol Modification and Thiol Proteomics\r......Page 389
Part 5_ Structural Proteomics......Page 403
21_The Utility of Mass Spectrometry Based\rStructural Proteomics in Biopharmaceutical\rBiologics Development\r......Page 405
Part 6_ Bioinformatics Tools......Page 423
22_nwCompare and AutoCompare Softwares for\rProteomics and Transcriptomics Data Mining –\rApplication to the Exploration of Gene\rExpression Profiles of Aggressive Lymphomas\r......Page 425
23_Application of Bioinformatics Tools\rin Gel-Based Proteomics\r......Page 435